WimAutophagy

Quantifies autophagosomes for inmunofluorescence autophagy assays

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Objective, reproducible, and precise analysis of autophagy assay images is ensured by our WimAutophagy online automated solution.

It is specifically designed to quantitatively monitor the autophagy process, by detecting the cell nuclei autophagosomes in the cytoplasm from cell or tissue cultures images.

You take the images, we do the rest.

About Cell

Autophagy represents the major process for degradation of aggregated cellular proteins and dysfunctional organelles, so alterations in autophagy are involved in the pathogenesis of many diseases, like nervous system disorders.

Thanks to our high-end image processing algorithms, WimAutophagy detects cell nuclei and allocates the autophagosomes to the corresponding nuclei, in order to obtain quantitative data per individual cell. It is also optimized to distinguish between isolated and clustered autophagosomes, in order to be able to estimate the number of autophagosomes and puncta per cell.

Results that make a difference

For every image that you upload, WimAutophagy will generate an output CSV sheet file that will give you insight into the outcome of your experiment. Some of the measurements included are:

  • Number of cells
  • Estimated autophagosome area and mean intensity
  • Mean number of puncta and autophagosomes per cell
  • Mean Nucleus area
  • Area of nucles, citoplasm and autophagosomes
  • Number of autophagosomes, by quantifying isolated and estimating clusters population

Besides, a detailed control image is generated in order to help you to visualize the parameters that WimAutophagy provides you with:

Negative cells
Positive Cells
Autophagosomes

Would you like to get any other parameter? Ask for it!

Discover the benefits of using WimAutophagy

There are many advantages of adding WimAutophagy to your workflow:

  • It is easy to use, fast and automated. Just upload your images and get your results in seconds.
  • Just pay for your number of images, not a cent more. WimAutophagy is a pay-per-use service.
  • Takes objective measurements with precision and accuracy.
  • It is able to work with many different types of microscopy techniques such as epifluorescence and confocal microscopy in adherent cell lines. If your image type is not covered, a custom solution can be developed upon request.
  • Suits for the reproducibility paradigm: same rules to measure the same kind of experiments.
  • Check your results from your Wimasis account anytime, anywhere. All you need is an Internet connection.

How it works

1
Upload your files
Try the WimApp that best fits you or request a Custom Solution.
2
Download your results
In the Results section you will have access to them in a few minutes.
3
Give us some feedback
We could tune our algorithms for you. It is free, just contact us!

See more about WimAutophagy

Here you can find some extra resources that will help you to fully understand this solution: