Evaluates cell viability in TdT-mediated dUTP nick end labeling (TUNEL) assays

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Objective, reproducible, and precise quantification of TUNEL assay images is ensured by our WimTUNEL online automated solution.

It generates meaningful results within minutes through the detection of the whole cell population inside the image and the identification of the apoptotic cells, marked with the TUNEL reagent, within it.

You take the images, we do the rest.

About TUNEL Assay

The study of the mechanisms that lead to DNA damage and their consequences is an invaluable source of information for a better understanding of human disease. The use of TdT-mediated dUTP nick end labeling (TUNEL) staining to assess this process is an extended practice in the study of apoptosis, necrosis and other DNA-damage related processes. Its effectiveness favors the screening of large batches of cell cultures with the aid of an image processing solution.

The study of transfection in cell cultures requires the production of a high number of transfected cells that need to be screened and monitored. The examination of the transfected cell population in a culture can become a tedious and time-consuming task, when performed by manual quantification. WimTransfection is specially designed to overcome this task fast and easily, providing you with objective and reliable results for as many images as you have.

Wimasis TUNEL assay uses as input fluorescence microscopy images with two different dyes: one nuclear or cytoplasmic membrane dye for the whole cell population and another marker for the TUNEL stain. If a cytoplasmic membrane dye is used, an extra nuclear dye can be applied to stain cellular nuclei, which will improve the accuracy of the cell detection algorithm.

Results that make a difference

For every image that you upload, WimTUNEL will generate an output CSV sheet file that will give you insight into the outcome of your experiment. Some of the measurements included are:

  • Total cell count: total number of cells in the image.
  • Count of Tunel-stained cells: total number of Tunel-stained cells in the image.
  • Tunel-stain ratio: percentage of Tunel-stained cells in the cell population.

Besides, a detailed control image is generated in order to help you to visualize the parameters that WimTUNEL provides you with:

Nucleus ID

Would you like to get any other parameter? Ask for it!

Discover the benefits of using WimTUNEL

There are many advantages of adding WimTUNEL to your workflow:

  • It is easy to use, fast and automated. Just upload your images and get your results in seconds.
  • Just pay for your number of images, not a cent more. WimTUNEL is a pay-per-use service.
  • Takes objective measurements with precision and accuracy.
  • Valid for all microscopy images, including unprocessed phase-contrast images with fluorescence.
  • Suits for the reproducibility paradigm: same rules to measure the same kind of experiments.
  • Check your results from your Wimasis account anytime, anywhere. All you need is an Internet connection.

How it works

Upload your files
Try the WimApp that best fits you or request a Custom Solution.
Download your results
In the Results section you will have access to them in a few minutes.
Give us some feedback
We could tune our algorithms for you. It is free, just contact us!

See more about WimTUNEL

Here you can find some extra resources that will help you to fully understand this solution:

Venoos Iman et al.

Anticancer and anti-inflammatory activities of girinimbine isolated from Murraya koenigii

Drug Design, Development and Therapy
January 2017