Evaluates the growth and structure of the new generated vessels in the chorioallantoic membrane

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Objective, reproducible, and precise quantification of CAM assay images is ensured by our WimCAM automated service.

Our image analysis solution is specifically developed to accurately analyze the growth and structure of the new generated vessels in the chorioallantoic membrane.

You take the images, we do the rest.

About CAM

The use of the chorioallantoic membrane (CAM) of developing chicken eggs has become a key practice to study angiogenesis and vascular processes in many vascular and developmental biomedical investigations. The simplicity of the CAM assay and its similarity to in vivo models makes it an interesting alternative to study the growth and damage of vessels and capillaries in vascular structures, which constitutes a target step in the study of tumors, cancer, angiogenesis, vascular diseases, developmental biology and an extensive number of other investigations.

The concordance of the CAM assay results with the in vivo data turns it into an engaging alternative to observe the evolution of the vascular structures with the promise of representative results. The changes on the vessels of the CAM and modifications of its vascular structure can be easily observed by the human eye; however, the quantification of those changes in an objective and reproducible way is only possible using image processing techniques. WimCAM is conceived to quantify CAM assay images automatically with precision and reliability.

WimCAM provides biological and biomedical researchers with objective and accurate measurements of the vascular structure of the CAM, such as the number of vessels and the vessel density of the membrane, which enables side-by-side comparison of different experiments.

WimCAM uses as input bright field microscopy images of the chorioallantoic membrane of chicken eggs. Both images taken from within the egg and images of a piece of the egg shell are suitable for the image analysis tool. Besides, our solution is engineered with the flexibility needed to fit other image kinds. So, if your CAM assay images do not fulfill the requirements above, don’t hesitate to contact us to get a trial for your images.

Results that make a difference

For every image that you upload, WimCAM will generate an output CSV sheet file that will give you insight into the outcome of your experiment. Some of the measurements included are:

  • Number of vessels segments
  • Number of branching points
  • Number of nets
  • Vessel density
  • Mean segment length
  • Total vessel network length

Besides, a detailed control image is generated in order to help you to visualize the parameters that WimCAM provides you with:

Region of Interest
Covered Area
Vessels segments
Branching Points

Would you like to get any other parameter? Ask for it!

Discover the benefits of using WimCAM

There are many advantages of adding WimCAM to your workflow:

  • It is easy to use, fast and automated. Just upload your images and get your results in seconds.
  • Just pay for your number of images, not a cent more. WimCAM is a pay-per-use service.
  • Takes objective measurements with precision and accuracy.
  • Valid for all microscopy images, including unprocessed phase-contrast images with fluorescence.
  • Suits for the reproducibility paradigm: same rules to measure the same kind of experiments.
  • Check your results from your Wimasis account anytime, anywhere. All you need is an Internet connection.

How it works

Upload your files
Try the WimApp that best fits you or request a Custom Solution.
Upload your files
Download your results
In the Results section you will have access to them in a few minutes.
Download results
Give us some feedback
We could tune our algorithms for you. It is free, just contact us!
Give us feedback

See more about WimCAM

Here you can find some extra resources that will help you to fully understand this solution: