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WimScratch
As one of the most commonly used assays in the field of cell migration, the Wound Healing Assay is conducted in order to study directional cell migration and cell-cell interaction in vitro. The basic procedure involves creating a "wound" in a cell monolayer, capturing images at regular intervals during cell migration until wound closure is reached, and the analysis of the image sequence in order to quantify the migration characteristics of these cells.

WimScratch, the Wound Healing Assay Image Analysis solution, is designed to generate objective and reproducible analysis results for Wound Healing Assays. The web-based automated solution generates results within minutes without the need to buy any extra software or hardware. Simply upload your assay images to our analysis platform and instantly receive detailed quantification for them.

You can find more information about WimScratch and the Wound Healing Assay by downloading our Product’s Detailed Description and Application Note on the right side of the page.
Scratch Assay image provided by the Department of Life Sciences, National Chung Hsing University, Taichung.
Citations
  • Nolwenn Briand, Anne-Claire Guénantin, Dorota Jeziorowska, Akshay Shah, Matthieu Mantecon, Emilie Capel, Marie Garcia, Anja Oldenburg, Jonas Paulsen, Jean-Sebastien Hulot, Corinne Vigouroux, Philippe Collas . The lipodystrophic hotspot lamin A p.R482W mutation deregulates the mesodermal inducer T/Brachyury and early vascular differentiation gene networks Human Molecular Genetics. February 2018
  • Muxing Kang , Wen Zheng , Qing Chen , Wenjie Qin , Pengping Li , Shifei Huang , Yizhao Zhou , Lantian Wang , Haolei Cai , Wenjie Lu , Biao Jiang , Qingqu Guo , Jian Chen , Dylan Wan , Jianyu Rao , Yulian Wu. Thymidylate synthase prompts metastatic progression through the dTMP associated EMT process in pancreatic ductal adenocarcinoma Cancer Letters. January 2018
  • Jing Hu, Li Bin Zhang, Qiang Chen, Jie Lin, Shao Bo Wang, Ri Liu, Wen Jing Zhang,Kun Miao, Tao Shou. Knockdown of CPEB4 expression suppresses cell migration and invasion via Akt pathway in non-small cell lung cancer Cell Biology International. December 2017
  • Bustamin Kosmo, Kee Wah Lee, Muhammad Sufyan bin Masroni, Sharne Leong, Wei Ern Ong, Hong Lyn Wong, Yi Yun Chang, Evelyn SC Koay, Sai Mun Leong2, Karen Tan. MicroRNA Profiling of Oxaliplatin-Resistant HCT116 Colorectal Cancer Cells Oncomedicine. July 2017
  • Cesar Bedoya, Andrés Cardona, July Galeano, Fabián Cortés-Mancera, Patrick Sandoz, Artur Zarzycki. Accurate Region-of-Interest Recovery Improves the Measurement of the Cell Migration Rate in the In Vitro Wound Healing Assay SLAS TECHNOLOGY: Translating Life Sciences Innovation. July 2017
  • Katayoon Pakravan, Sadegh Babashah, Majid Sadeghizadeh, Seyed Javad Mowla, Majid Mossahebi-Mohammadi, Farangis Ataei, Nasim Dana, Mohammad Javan. MicroRNA-100 shuttled by mesenchymal stem cell-derived exosomes suppresses in vitro angiogenesis through modulating the mTOR/HIF-1?/VEGF signaling axis in breast cancer cells Cellular Biology. July 2017
  • Mohammad Amin Mahjoub, Babak Bakhshinejad, Majid Sadeghizadeh, Sadegh Babashah. Combination treatment with dendrosomal nanocurcumin and doxorubicin improves anticancer effects on breast cancer cells through modulating CXCR4/NF-?B/Smo regulatory network Molecular Biology Reports. July 2017
  • Wei-Lun Liu, Jia-Ming Chang, Inn-Wen Chong, Yi-Li Hung, Yung-Hsiang Che, Wen-Tsung Huang, Hsuan-Fu Kuo, Chong-Chao Hsieh and Po-Len Liu. Curcumin Inhibits LIN-28A through the Activation of miRNA-98 in the Lung Cancer Cell Line A549 Molecules. June 2017
  • Ang Gao, Ruiqiang Hang, Wan Li, Wei Zhang, Penghui Li, Guomin Wang, Long Bai, Xue-Feng Yu, Huaiyu Wang, Liping Tong, Paul K. Chu. Linker-free covalent immobilization of heparin, SDF-1?, and CD47 on PTFE surface for antithrombogenicity, endothelialization and anti-inflammation Biomaterials. June 2017
  • Al Hassan Kyakulaga. Withaferin a synergistically enhances the effects of paclitaxel against lung cance r PhD Thesis - University of Louisville. May 2017
  • View all
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Please, choose the quantity
  • 100 images 125.00 €
  • 250 images 250.00 €
  • 500 images 350.00 €
  • 1000 images 500.00 €
  • 2000 images 900.00 €
  • 5000 images 2,000.00 €
  • 10000 images 3,500.00 €
  • 20000 images 6,000.00 €
(Tax free)
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Analysis data contain
  • ✔ Cell covered area
  • ✔ Overview chart
  • ✔ Center piece approximation
  • ✔ Speed of closure
  • ✔ Acceleration characteristics
Would you like to get any other parameter? Ask for it!
Case studies
The Ludwig-Maximilians-University (LMU) Munich
Center for Drug Research, Chair of Pharmaceutical Biology (Prof. Angelika Vollmar)
The Center for Drug Research covers all research areas around the development of novel drug – from chemical synthesis to innovative formulations. At the Chair of Pharmaceutical Biology (Prof. Dr. Angelika Vollmar) we investigate the pharmaceutical potential of natural compounds, be it of plant, animal or microbial origin.

Our main focus lies on tumor biology, with tumor angiogenesis (Prof. Dr. Stefan Zahler) as a central topic. Inhibiting the vessel growth in tumors in order to “starve” the malignancy is an old therapeutic concept, which has led to some clinical success, but still has its limits: due to evasive strategies of the tumors and to development of resistances we are still in need of novel therapeutic targets and inhibitors of angiogenesis.
Therefore, not only try to get a better academic understanding of the angiogenesis process, but also screen novel inhibitors. More complex angiogenesis assays, like the tube formation assay, are image based and tedious to evaluate by hand.

Therefore they often are of limited use for screening purposes. The WimTube module overcomes this problem: it enables a robust rational evaluation of tube formation assays, and allows us to use this assay in a medium throughput screening approach.
In addition to the highly reproducible analysis, we also get valuable morphological information, which has to date not been used in classical evaluation routines.
View case study
Aksaray University
Department of Biology, Faculty of Science and Letters
Our main scientific interest is to combine biology and nanotechnology, meeting in cancer studies using multifunctional nanoparticles. Wimasis enables us to measure both in vitro scratch assay and in-ovo CAM assay by giving results in a shorter time and more standardized manner. Initial and final scratch sizes are determined using the WimScratch analysis tool and the difference between the two is used to determine migration distance using ratio of scratch area to cell covered area.

We also frequently use CAM model in angiogenesis studies. Instead of using the macroscopic scoring method, thanks to WimCAM we can reliably measure many parameters such as vesseldensity, total vessel network length, total branching points, total nets, and segment properties, hidden details in the images obtained after the nanomaterials-biological system interaction in CAM assay, thus we can make comparisons between these measurements in a short time. It also allows seeing measurements on the processed image after all these analyzes and makes you evaluate measurement quality. By this way, we cannot only save time but also get more reliable morphological information than classical methods.

As it is known, this is crucial for a better understanding of the relationship between cells/tissues and nano-sized structures and for making way for successful clinical applications. Thanks to Wimasis’ online tools, it is now possible to make more informative analyzes about interactions between nanomaterials and biological systems.
View case study
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