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Quantification of the wound healing assay is a straightforward method to study cell migration in vitro

WimScratch does it for you in seconds
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Objective, reproducible, and precise quantification of wound healing assay images is ensured by our WimScratch online automated solution.

It is engineered to monitor the wound closing process by detecting cell and wound area, with the aim to provide you with time and spatial results of your assay in seconds.

You take the images, we do the rest.

Analyzing the scratch assay

As one of the most commonly used assays in the field of cell migration, the wound healing or scratch assay is conducted in order to study directional cell migration and cell-cell interaction in vitro. The basic procedure involves creating a "wound" in a cell monolayer, capturing images at regular intervals during cell migration until wound closure is reached, and the analysis of the image sequence in order to quantify the migration characteristics of these cells.

WimScratch, the Wound Healing Assay Image Analysis solution, is designed to generate accurate and reproducible analysis results for wound healing assays, both for phase contrast and fluorescence images. The online automated solution generates results within seconds without the need to buy any extra software or hardware. Simply upload your assay images to our analysis platform and instantly receive detailed quantification for them.

You can find further information about WimScratch and the wound healing assay here

Discover the benefits of using WimScratch

More than 1800 researchers already use WimScratch in their research work. These are the main reasons why they choose it:

  • It is easy to use, fast and automated. Just upload your images and get your results in seconds.
  • Just pay for your number of images, not a cent more. WimScratch is a pay-per-use service.
  • Takes objective measurements with precision and accuracy.
  • Suits for the reproducibility paradigm: same rules to measure the same kind of experiments.
  • Check your results from your Wimasis account anytime, anywhere. All you need is an Internet connection.

How it works

That is simple! Just go to myWim, Wimasis' Image Analysis Platform, and follow these steps:

join us
Create an account
upload images
Upload your images
Enjoy the results

Would you like to know more about Wimasis Cloud? Please, click here for further information.

Results that make a difference

For every image that you upload, WimScratch will generate an output CSV sheet file that will give you insight into the outcome of your experiment. Some of the measurements included are:

  • Cell covered area
  • Overview chart
  • Center piece approximation
  • Speed of closure
  • Acceleration characteristics

Besides, a detailed control image is generated in order to help you to visualize the parameters that WimScratch provides you with:

Covered area

Would you like to get any other parameter? Ask for it!

See more about WimScratch

Here you can find some extra resources that will help you to fully understand this solution:

Nikoleta F. Theodoroula et al.
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