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Objective, reproducible, and precise quantification of comet assay fluorescence images is ensured by our WimComet web-based automated solution.
Its high-end image processing algorithms are engineered to measure DNA damage of multiple comets in each image individually by comparing the absolute and relative signal of their head and tail.
You take the images, we do the rest.
The comet assay is an extended method for the detection of DNA damage. Its simplicity, rapidness and reliability make it ideal for a wide variety of experiments where the study of DNA damage is the target. Some experiments where the comet assay is generally used are the study of genotoxicity in the screening of new drug compounds, the monitoring of environmental contamination with genotoxins and the research of fundamental DNA damage and repair.
The easiness of the comet assay combined with its reliability makes of it one of the most interesting and extended techniques to measure DNA damage. The DNA damage can be easily observed in the comet-like structure, but not so easily quantified. The use of image processing and analysis is the key to get objective and reproducible data to help you measure objectively the DNA damage in your comet assay. WimComet is conceived to provide you with that objective quantification with accuracy and reproducibility.
There are many advantages of adding WimComet to your workflow:
For every image that you upload, WimComet will generate an output CSV sheet file that will give you insight into the outcome of your experiment. Some of the measurements included are:
Besides, a detailed control image is generated in order to help you to visualize the parameters that WimComet provides you with:
Would you like to get any other parameter? Ask for it!
Here you can find some extra resources that will help you to fully understand this solution: