Lipid droplets are fundamental in regulating the storage of neutral lipids, that could be accessed according to
metabolic request. But they are not only energy depots, as this dynamic organelles form part of many biological
processes like cellular energy homeostasis and lipid metabolism. The lipid droplets assay is used, therefore, in
research of metabolic diseases, such as obesity, diabetes and atheroscleros.
WimLipid is our image analysis solution specially developed to quantify objectively the proliferation, growth
and distribution of lipid droplets, providing reliable data such as the number of these organelles, their covered
area or the distribution of areas in intervals.
WimLipid uses as inputs brigthfield, phase contrast or fluorescence microscopy images of lipid droplets assay images. Lipid
droplets could appear refractile or stained, as shown in the next image. Optionally, cells can be stained with
nuclear dyes (such as the DAPI or Hoechst), which will be helpful to provide extra parameters of the analysis.
Lipid Droplets image granted by the ZIEL Molecular Nutritional Medicine, Technische Universität München.