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Objective, reproducible, and precise quantification of TUNEL assay images is ensured by our WimTUNEL online automated solution.
It generates meaningful results within minutes through the detection of the whole cell population inside the image and the identification of the apoptotic cells, marked with the TUNEL reagent, within it.
You take the images, we do the rest.
The study of the mechanisms that lead to DNA damage and their consequences is an invaluable source of information for a better understanding of human disease. The use of TdT-mediated dUTP nick end labeling (TUNEL) staining to assess this process is an extended practice in the study of apoptosis, necrosis and other DNA-damage related processes. Its effectiveness favors the screening of large batches of cell cultures with the aid of an image processing solution.
The study of transfection in cell cultures requires the production of a high number of transfected cells that need to be screened and monitored. The examination of the transfected cell population in a culture can become a tedious and time-consuming task, when performed by manual quantification. WimTransfection is specially designed to overcome this task fast and easily, providing you with objective and reliable results for as many images as you have.
Wimasis TUNEL assay uses as input fluorescence microscopy images with two different dyes: one nuclear or cytoplasmic membrane dye for the whole cell population and another marker for the TUNEL stain. If a cytoplasmic membrane dye is used, an extra nuclear dye can be applied to stain cellular nuclei, which will improve the accuracy of the cell detection algorithm.
There are many advantages of adding WimTUNEL to your workflow:
For every image that you upload, WimTUNEL will generate an output CSV sheet file that will give you insight into the outcome of your experiment. Some of the measurements included are:
Besides, a detailed control image is generated in order to help you to visualize the parameters that WimTUNEL provides you with:
Would you like to get any other parameter? Ask for it!
Here you can find some extra resources that will help you to fully understand this solution: