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The formation of new blood vessels is essential in growth and development of biological systems. The research focus of many drug screening and cell signaling studies is aiming at the interaction of initial and continuous growth and sustentation of solid tumors and its complex physiological processes due to angiogenic activity.

The in vitro Tube Formation Assay is based on the differentiation of endothelial cells and the formation of tube-like structures on an extracellular matrix such as Matrigel® (BD).

This assay can be conducted to screen compounds for angiogenic activity and is able to deliver good, reproducible results. The major advantage of this assay is that it offers the opportunity to monitor angiogenic behavior over time and therefore enabling the estimation of time dependent effects on the neovascularization.

The objective, reliable, and precise quantification of Tube Formation images is ensured by the Wimasis WimTube Solution, without the need for extra hardware or software. WimTube is engineered with flexibility to adapt to all kind of images, so it accepts images of the whole range of microscopy techniques used in tube formation assay with the aim to provide every researcher with the automated analysis that best fits his needs.
Tube Formation image sample provided by the Institute of Biomedicine (IBIOMED) of the University of León, Spain.
  • Federico Galvagni,, Federica Nardi, Ottavia Spiga, Alfonso Trezza, Giulia Tarticchio, Rosanna Pellicani, Eva Andreuzzi, Elena Caldi, Paolo Toti, Gian Marco Tosi, Annalisa Santucci. Dissecting the CD93-Multimerin 2 interaction involved in cell adhesion and migration of the activated endothelium Matrix Biology. September 2017
  • Chih-Jen Lai, Hsu-Chen Cheng, Ching-Yu Lin, Shih-Han Huang, Ting-Huan Chen, Chi-Jung Chung, Chung-Ho Chang, Horng-Dar Wang and Chih-Pin Chuu. Activation of liver X receptor suppresses angiogenesis via induction of ApoD The FASEB Journal. August 2017
  • Amy K. L. Chui, Tilini N. Gunatillake, Vera Ignjatovic, Paul T. Monagle, Padma Murthi, Shaun P. Brennecke, John M. Whitelock and Joanne M. Said. Antiangiogenic effects of decorin restored by unfractionated, low molecular weight, and nonanticoagulant heparins Blood Advances. July 2017
  • TDK Herath, Anis Larbi, Swee Hin Teoh, C. James Kirkpatrick, Bee Tin Goh. Neutrophil-mediated enhancement of angiogenesis and osteogenesis in a novel triple cell co-culture model with endothelial cells and osteoblasts Tissue Engineering and Regenerative Medicine. July 2017
  • Peng Yu, Shengjie Li, Zhifei Zhang, Xiaolong Wen, Wei Quan, Qilong Tian, Chuang Gao, Wanqiang Su, Jianning Zhang, Rongcai Jiang. Progesterone-mediated angiogenic activity of endothelial progenitor cell and angiogenesis in traumatic brain injury rats were antagonized by progesterone receptor antagonist Cell Proliferation. July 2017
  • Sinem Tunçer, Ay?e Gökçe Ke?kü?, Melis Çolako?lu, Ismail Çimen, 1, Caner Yener, Özlen Konu, Sreeparna Banerjee. 15-Lipoxygenase-1 re-expression in colorectal cancer alters endothelial cell features through enhanced expression of TSP-1 and ICAM-1 Cellular Signalling. July 2017
  • Chang Chen, Qi Tang, Yan Zhang, Minjia Dai, Yichen Jiang, Hang Wang, Mei Yu, Wei Jing, Weidong Tian. Metabolic reprogramming by HIF-1 activation enhances survivability of human adipose-derived stem cells in ischaemic microenvironments Cell Proliferation. July 2017
  • Marek Kukumberga, Jia Yi Yaob, Dawn J.H. Neoa, Evelyn K.F. Yima. Microlens topography combined with vascular endothelial growth factor induces endothelial differentiation of human mesenchymal stem cells into vasculogenic progenitors Biomaterials. July 2017
  • Francisca Bahamondes, Estefania Flores, Gino Cattaneo, Flavia Bruna and Paulette Conget. Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells BMC Veterinary Research . June 2017
  • Shih -Chieh Lin, Hsiu -Chi Lee , Pei -Chi Hou, Jhao -Lin Fu, Meng -Hsing Wu and Shaw -Jenq Tsai. Targeting hypoxia-mediated YAP1 nuclear translocation ameliorates pathogenesis of endometriosis without compromising maternal fertility The Journal Of Pathology. June 2017
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Please, choose the quantity
  • 100 images 250.00 €
  • 250 images 500.00 €
  • 500 images 750.00 €
  • 1000 images 1,000.00 €
  • 2000 images 1,700.00 €
  • 5000 images 4,000.00 €
  • 10000 images 7,000.00 €
  • 20000 images 12,000.00 €
(Tax free)
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Analysis data contain
  • ✔ Branching points
  • ✔ Tube lengths, numbers, and statistics
  • ✔ Loop numbers, areas, and perimeters
  • ✔ Cell-covered area
Would you like to get any other parameter? Ask for it!
Case studies
The Ludwig-Maximilians-University (LMU) Munich
Center for Drug Research, Chair of Pharmaceutical Biology (Prof. Angelika Vollmar)
The Center for Drug Research covers all research areas around the development of novel drug – from chemical synthesis to innovative formulations. At the Chair of Pharmaceutical Biology (Prof. Dr. Angelika Vollmar) we investigate the pharmaceutical potential of natural compounds, be it of plant, animal or microbial origin.

Our main focus lies on tumor biology, with tumor angiogenesis (Prof. Dr. Stefan Zahler) as a central topic. Inhibiting the vessel growth in tumors in order to “starve” the malignancy is an old therapeutic concept, which has led to some clinical success, but still has its limits: due to evasive strategies of the tumors and to development of resistances we are still in need of novel therapeutic targets and inhibitors of angiogenesis.
Therefore, not only try to get a better academic understanding of the angiogenesis process, but also screen novel inhibitors. More complex angiogenesis assays, like the tube formation assay, are image based and tedious to evaluate by hand.

Therefore they often are of limited use for screening purposes. The WimTube module overcomes this problem: it enables a robust rational evaluation of tube formation assays, and allows us to use this assay in a medium throughput screening approach.
In addition to the highly reproducible analysis, we also get valuable morphological information, which has to date not been used in classical evaluation routines.
View case study
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