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The formation of new blood vessels is essential in growth and development of biological systems. The research focus of many drug screening and cell signaling studies is aiming at the interaction of initial and continuous growth and sustentation of solid tumors and its complex physiological processes due to angiogenic activity.

The in vitro Tube Formation Assay is based on the differentiation of endothelial cells and the formation of tube-like structures on an extracellular matrix such as Matrigel® (BD).

This assay can be conducted to screen compounds for angiogenic activity and is able to deliver good, reproducible results. The major advantage of this assay is that it offers the opportunity to monitor angiogenic behavior over time and therefore enabling the estimation of time dependent effects on the neovascularization.

The objective, reliable, and precise quantification of Tube Formation images is ensured by the Wimasis WimTube Solution, without the need for extra hardware or software. WimTube is engineered with flexibility to adapt to all kind of images, so it accepts images of the whole range of microscopy techniques used in tube formation assay with the aim to provide every researcher with the automated analysis that best fits his needs.
Tube Formation image sample provided by the Institute of Biomedicine (IBIOMED) of the University of León, Spain.
  • Kinga G. Blecharz-Lang, Josephin Wagner, Alexa Fries, Melina Nieminen-Kelhä, Jörg Rösner, Ulf C. Schneider, Peter Vajkocz. Disturbances Can Be Attenuated by Blockade of the IL6 Receptor Expressed in Brain Microvascular Endothelial Cells Translational Stroke Research. February 2018
  • Xinyan Miao, Mohammed Ferdous - Ur Rahman, Lei Jia ng, Yanan Min, Shuai Tan, Hong Xie, Linkiat Lee, Miao Wang, Rickard E. Malmström, Weng - Onn Lui, Nailin Li. Thrombin-reduced miR-27b attenuates platelet angiogenic activities in vitro via enhancing platelet synthesis of anti-angiogenic thrombospondin-1 Journal of Thrombosis and Haemostasis. February 2018
  • Chai San Ho, Suzita Mohd Noor, Noor Hasima Nagoor. MiR -378 and MiR -1827 Regulate Tumor Invasion, Migration and Angiogenesis in Human Lung Adenocarcinoma by Targeting RBX1 and CRKL , Respectively Journal of Cancer. January 2018
  • E. Bauman, T. Feijão, D. T. O. Carvalho, P. L. Granja & C. C. Barrias. Xeno-free pre-vascularized spheroids for therapeutic applications Scientific Reports. January 2018
  • Francisco Javier Rodríguez-Baena, Silvia Redondo-García, María del Carmen Plaza-Calonge, Rubén Fernández-Rodríguez, Juan Carlos Rodríguez-Manzaneque. Evaluation of Tumor Vasculature Using a Syngeneic Tumor Model in Wild-Type and Genetically Modified Mice Proteases and Cancer. January 2018
  • Y. An, W. J. Liu, P. Xue, Y. Ma, L. Q. Zhang, B. Zhu, M. Qi, L. Y. Li, Y. J. Zhang, Q. T. Wang & Y. Jin. Autophagy promotes MSC-mediated vascularization in cutaneous wound healing via regulation of VEGF secretion Cell Death & Disease. January 2018
  • Kaela M. Varberg , Seth Winfree , Kenneth W. Dunn , Laura S. Haneline. Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro Journal of Visualized Experiments. January 2018
  • Marianne Schmidt, Josef Skaf, Georgiana Gavril, Christine Polednik, Jeanette Roller, Michael Kessler and Ulrike Holzgrabe. The influence of Osmunda regalis root extract on head and neck cancer cell proliferation, invasion and gene expression BMC Complementary and Alternative Medicine. December 2017
  • Mingming Liu, Xiaoyan Zhang, Bing Wang, Qingbin Wu, Bingwei Li, Ailing Li, Honggang Zhang & Ruijuan Xiu. Functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat Scientific Reports. December 2017
  • Emily M. dos Reis, Fernanda V. Berti, Guilherme Colla, Luismi M. Porto. Bacterial nanocellulose-IKVAV hydrogel matrix modulates melanoma tumor cell adhesion and proliferation and induces vasculogenic mimicry in vitro Journal of Biomedical Materials Research . December 2017
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Please, choose the quantity
  • 100 images 250.00 €
  • 250 images 500.00 €
  • 500 images 750.00 €
  • 1000 images 1,000.00 €
  • 2000 images 1,700.00 €
  • 5000 images 4,000.00 €
  • 10000 images 7,000.00 €
  • 20000 images 12,000.00 €
(Tax free)
Custom solution? Contact us
Analysis data contain
  • ✔ Branching points
  • ✔ Tube lengths, numbers, and statistics
  • ✔ Loop numbers, areas, and perimeters
  • ✔ Cell-covered area
Would you like to get any other parameter? Ask for it!
Case studies
The Ludwig-Maximilians-University (LMU) Munich
Center for Drug Research, Chair of Pharmaceutical Biology (Prof. Angelika Vollmar)
The Center for Drug Research covers all research areas around the development of novel drug – from chemical synthesis to innovative formulations. At the Chair of Pharmaceutical Biology (Prof. Dr. Angelika Vollmar) we investigate the pharmaceutical potential of natural compounds, be it of plant, animal or microbial origin.

Our main focus lies on tumor biology, with tumor angiogenesis (Prof. Dr. Stefan Zahler) as a central topic. Inhibiting the vessel growth in tumors in order to “starve” the malignancy is an old therapeutic concept, which has led to some clinical success, but still has its limits: due to evasive strategies of the tumors and to development of resistances we are still in need of novel therapeutic targets and inhibitors of angiogenesis.
Therefore, not only try to get a better academic understanding of the angiogenesis process, but also screen novel inhibitors. More complex angiogenesis assays, like the tube formation assay, are image based and tedious to evaluate by hand.

Therefore they often are of limited use for screening purposes. The WimTube module overcomes this problem: it enables a robust rational evaluation of tube formation assays, and allows us to use this assay in a medium throughput screening approach.
In addition to the highly reproducible analysis, we also get valuable morphological information, which has to date not been used in classical evaluation routines.
View case study
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