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Aksaray University
Department of Biology, Faculty of Science and Letters
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WimCAM allows us to analyze vascularization quantitatively and reliably in CAM assay as an alternative angiogenesis model system.
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Dr. Gamze Tan
Research scientist, Department of Biology, Aksaray University
Our main scientific interest is to combine biology and nanotechnology, meeting in cancer studies using multifunctional nanoparticles. Wimasis enables us to measure both in vitro scratch assay and in-ovo CAM assay by giving results in a shorter time and more standardized manner. Initial and final scratch sizes are determined using the WimScratch analysis tool and the difference between the two is used to determine migration distance using ratio of scratch area to cell covered area.

We also frequently use CAM model in angiogenesis studies. Instead of using the macroscopic scoring method, thanks to WimCAM we can reliably measure many parameters such as vesseldensity, total vessel network length, total branching points, total nets, and segment properties, hidden details in the images obtained after the nanomaterials-biological system interaction in CAM assay, thus we can make comparisons between these measurements in a short time. It also allows seeing measurements on the processed image after all these analyzes and makes you evaluate measurement quality. By this way, we cannot only save time but also get more reliable morphological information than classical methods.

As it is known, this is crucial for a better understanding of the relationship between cells/tissues and nano-sized structures and for making way for successful clinical applications. Thanks to Wimasis’ online tools, it is now possible to make more informative analyzes about interactions between nanomaterials and biological systems.
Products they are using
Centro de Investigaciones Biológicas (CIB-CSIC)
Autophagy in Development and Disease
Our lab uses cellular and animal models to understand the physiological roles of autophagy and its implications during disease.

Autophagy is an essential intracelullar degradation pathway that recycles cell components generating new building blocks and energy to maintain cellular homeostasis. Autophagy plays an important role in the response to nutrient starvation; the recycling of damaged organelles and is a survival mechanism under stress conditions. In addition, autophagy could as well participate in programmed cell death.

We are interested in the implication of autophagy during development and in the relationship of autophagy with basic processes such as proliferation, differentiation and cell death. Moreover we want to understand how autophagy deregulation may play a role in several pathological situations such as cancer and neurodegenerative conditions.

We have several projects with pharmaceutical companies to screen for new drugs that modulate autophagy with the aim to find new treatments for cancer, neurodegenerative diseases and other pathological conditions.

Wimasis has allowed us to speed up and standardize the process of autophagosome quantification in cells and tissues.
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University of Córdoba
Department of Cell Biology Physiology and Immunology, Faculty of Sciences
In this case we study alterations in mitochondrial ultrastructure and dynamics in kidney cells of mice submitted to calorie restriction. It has been proofed in a wide range of animal species, that caloric restriction without malnutrition (RC) is the most robust experimental intervention that increases longevity and delays the onset of cancer, kidney disease, cataracts, diabetes, hypertension, etc... Although the extension of the maximum longevity has not been fully tested in primates, other beneficial effects accepted that this intervention occurs in these animals and in humans. However, it has not been fully elucidated the mechanisms through which the RC operates to produce these effects.

Currently "Theory of Free Radicals” propose that the cellular aging occurs due to the accumulation of reactive oxygen species (ROS), this theory appears to be the most logical to explain the effects of the RC, because as it has been shown, that the intervention RC drastically decreases the production of ROS in cells, supporting the idea that a decrease in oxidative stress may be a mechanism that contributes to delay aging. Moreover, it has been found that it is the organelle mitochondria where the increased production and accumulation of ROS occurs, and thus constitutes a special target for studies on aging and caloric restriction. Furthermore, numerous alterations have been detected in mitochondria during aging (reduction of mitochondrial biogenesis and ATP synthesis, increased leakage of H + , etc.), many of which are reversed when the animals are subjected to periods of RC.

Other studies have found an inverse correlation between longevity and the degree of unsaturation of membrane phospholipids , having assumed that polyunsaturated fatty acids are more susceptible to lipid peroxidation and other changes that would result in the accumulation of ROS in the cells. Finding least amount of polyunsaturated fatty acids after CR periods support this idea. Therefore, it is possible to assume that the decrease in the number of double bonds in fatty acids in the membranes can be an adaptation of the longest species to prevent the development and accumulation of oxidative damage.

Previous studies conducted in our group have shown that CR produces alterations in the ultrastructure and dynamics of fission / fusion of mouse liver mitochondria, and these effects are modulated by dietary fat composition in CR . However, it is completely unknown whether alterations in kidney mitochondria, specifically in cells of the proximal convoluted tubule, a structure that plays a key role in the reabsorption of molecules and electrolytes and therefore the proper functioning of the body, which physiology also altered during aging.

In cooperation with Wimasis we were able to develop an automatic method for automatic detection of mitochondria in kidney tissue and isolated monocyte cells, providing us with thousands of planimetrics and stereologycs parameters in a record time. Wimasis “Stereology” software makes the improvement of data recompilation possible, in a fast,and reliable way, and ensuring the reproducibility of our material.
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