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Quantifying Tube Formation is a crucial step in angiogenesis assays.

WimTube does it for you in seconds.
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Objective, reproducible, and precise quantification of Tube Formation images is ensured by our WimTube online automated solution.

It is engineered with flexibility to adapt to images covering the whole range of microscopy imaging techniques with the aim to provide you with accurate, meaningful results.

You take the images, we do the rest.

Analyzing the tube formation assay

The formation of new blood vessels is essential for the growth, development and regeneration of biological systems as well as in certain diseases like cancer, where tumors stimulate this process in order to support their growth. The study of how drug compounds can promote or inhibit it is therefore essential in the research work related to these processes.

The tube formation assay studies blood vessel development by measuring the angiogenesis stage of endothelial cell reorganization to form tube or capillary-like structures, usually using an extracellular matrix such as Matrigel® (BD) as support. The major advantage of this assay is that it offers the opportunity to monitor angiogenic behavior over time and therefore enables the estimation of time dependent effects on the neovascularization.

Discover the benefits of using WimTube

More than 2000 researchers already use WimTube in their research work. These are the main reasons why they choose it:

  • It is easy to use, fast and automated. Just upload your images and get your results in seconds. No extra hardware or software needed.
  • Just pay for your number of images, not a cent more. WimTube is a pay-per-use service.
  • Valid for all microscopy images, including unprocessed phase-contrast images with fluorescence.
  • Takes objective measurements with precision and accuracy.
  • Suits for the reproducibility paradigm: same rules to measure the same kind of experiments.
  • Check your results from your Wimasis account anytime, anywhere. All you need is an Internet connection.

How it works

That is simple! Just go to myWim, Wimasis' Image Analysis Platform, and follow these steps:

join us
Create an account
upload images
Upload your images
Enjoy the results

Would you like to know more about Wimasis Cloud? Please, click here for further information.

Results that make a difference

For every image that you upload, WimTube will generate an output CSV sheet file that will give you insight into the tube formation process of your experiment. Some of the measurements included are:

  • Branching points
  • Tube lengths, numbers, and statistics
  • Loop numbers, areas, and perimeters
  • Cell-covered area

Besides, a detailed control image is generated in order to help you to visualize the parameters that WimTube provides you with:

Covered Area
Branching Points

Would you like to get any other parameter? Ask for it!

See more about WimTube

Here you can find some extra resources that will help you to fully understand this solution:

Lei Bao et al.
SAP30 promotes breast tumor progression by bridging the transcriptional corepressor SIN3 complex and MLL1
Journal of Clinical Investigation
September 2023
View all
case study
The Ludwig-Maximilians-University (LMU) Munich
The Ludwig-Maximilians-University (LMU) Munich DE
With the help of Wimasis we can really do quantitative analysis of image-based angiogenesis assays
Prof. Dr. Stefan Zahler
View case study
pie circulos
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