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The comet assay or SCGE is the standard for single-cell DNA damage evaluation

Let WimComet handle it for you
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Objective, reproducible, and precise quantification of comet assay fluorescence images is ensured by our WimComet online automated solution.

Its high-end image processing algorithms are engineered to measure DNA damage of multiple comets in each image individually by comparing the absolute and relative signal of their head and tail.

You take the images, we do the rest.

Analyzing the comet assay

The comet assay is an extended method for the detection of DNA damage. Its simplicity, rapidness and reliability make it ideal for a wide variety of experiments where the study of DNA damage is the target. Some experiments where the comet assay is generally used are the study of genotoxicity in the screening of new drug compounds, the monitoring of environmental contamination with genotoxins and the research of fundamental DNA damage and repair.

The easiness of the comet assay combined with its reliability makes of it one of the most interesting and extended techniques to measure DNA damage. The DNA damage can be easily observed in the comet-like structure, but not so easily quantified. The use of image processing and analysis is the key to get objective and reproducible data to help you measure objectively the DNA damage in your comet assay. WimComet is conceived to provide you with that objective quantification with accuracy and reproducibility.

Discover the benefits of using WimComet

There are many advantages of adding WimComet to your workflow:

  • It is easy to use, fast and automated. Just upload your images and get your results in minutes.
  • Just pay for your number of images, not a cent more. WimComet is a pay-per-use service.
  • It is independent of the electrophoresis direction. We adapt to your images, not the other way around.
  • Takes objective measurements with precision and accuracy.
  • Suits for the reproducibility paradigm: same rules to measure the same kind of experiments.
  • Check your results from your Wimasis account anytime, anywhere. All you need is an Internet connection.

How it works

That is simple! Just go to myWim, Wimasis' Image Analysis Platform, and follow these steps:

join us
Create an account
upload images
Upload your images
Enjoy the results

Would you like to know more about myWim? Please, click here for further information.

Results that make a difference

For every image that you upload, WimComet will generate an output CSV sheet file that will give you insight into the outcome of your experiment. Some of the measurements included are:

  • Total comet intensity
  • Comet length
  • Percentage of DNA in the tail
  • Tail length
  • Olive tail moment

Besides, a detailed control image is generated in order to help you to visualize the parameters that WimComet provides you with:

Comet's head
Comet's tail
Comet ID

Would you like to get any other parameter? Ask for it!

See more about WimComet

Here you can find some extra resources that will help you to fully understand this solution:

Michael Byrne, Richard L. Bennett, Xiaodong Cheng, W. Stratford May
Progressive Genomic Instability in the Nup98-HoxD13 Model of MDS Correlates with Loss of the PIG-A Gene Product
August 2014
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