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Analysis of the proliferation pattern in CFC assays by eye can render inaccurate results

Get precise quantitative data with WimColony
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Objective, reproducible, and precise quantification of colony forming cell (CFC) assays is ensured by our WimColony online automated solution.

WimColony works by detecting each individual cell and colony, followed by the extraction of their identifying characteristics in order to provide reliable data about the colony population and its development.

You take the images, we do the rest.

Analyzing the colony forming cell assay

The colony forming cell assay is a frequently used technique to measure the ability of cells to grow, monitoring the formation of colonies from an initially known cell population. The formation of colonies requires intensive division of the original cells, so the quantification of the number of cells or colonies throughout the experiment constitutes the perfect indicator of the growth potential of cells. As a result, the CFC has become an assay of extensive use in cancer research to study the effects of drugs and the ionizing radiation therapy in carcinogenic cell proliferation.

The observation of the colony forming process and development by the human eye is a practical method to estimate the overall evolution of the assay, but an ineffective way to accurately compare the proliferation activity of large cell culture populations or multiple assays at a time. WimColony is specially made to provide you the objective data you need to make the most of your research.

Discover the benefits of using WimColony

There are many advantages of adding WimColony to your workflow:

  • It is easy to use, fast and automated. Just upload your images and get your results in minutes.
  • Just pay for your number of images, not a cent more. WimColony is a pay-per-use service.
  • Works with input phase contrast, bright field and fluorescence microscopy images of cell colonies in any stage of the colony forming process. If your image type is not covered, a custom solution can be developed upon request.
  • Takes objective measurements with precision and accuracy.
  • Suits for the reproducibility paradigm: same rules to measure the same kind of experiments.
  • Check your results from your Wimasis account anytime, anywhere. All you need is an Internet connection.

How it works

That is simple! Just go to myWim, Wimasis' Image Analysis Platform, and follow these steps:

join us
Create an account
upload images
Upload your images
Enjoy the results

Would you like to know more about myWim? Please, click here for further information.

Results that make a difference

For every image that you upload, WimColony will generate an output CSV sheet file that will give you insight into the outcome of your experiment. Some of the measurements included are:

  • Number of detected objects
  • Mean object area
  • Single object area

Besides, a detailed control image is generated in order to help you to visualize the parameters that WimColony provides you with:

Colony outline
Colony ID

Would you like to get any other parameter? Ask for it!

See more about WimColony

Here you can find some extra resources that will help you to fully understand this solution:

Vernon J.Ebegboni, Reham M.Balahmar, John M.Dickenson, Shiva D.Sivasubramaniam
The effects of flavonoids on human first trimester trophoblast spheroidal stem cell self-renewal, invasion and JNK/p38 MAPK activation: Understanding the cytoprotective effects of these phytonutrients against oxidative stress
Biochemical Pharmacology
June 2019
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