The use of the chorioallantoic membrane (CAM) of developing chicken eggs has become a key practice to study angiogenesis and vascular processes in many vascular and developmental biomedical investigations. The simplicity of the CAM assay and its similarity to in vivo models makes it an interesting alternative to study the growth and damage of vessels and capillaries in vascular structures, which constitutes a target step in the study of tumors, cancer, angiogenesis, vascular diseases, developmental biology and an extensive number of other investigations.

The concordance of the CAM assay results with the in vivo data turns it into an engaging alternative to observe the evolution of the vascular structures with the promise of representative results. The changes on the vessels of the CAM and modifications of its vascular structure can be easily observed by the human eye; however, the quantification of those changes in an objective and reproducible way is only possible using image processing techniques. WimCAM is conceived to quantify CAM assay images automatically with precision and reliability.

WimCAM provides biological and biomedical researchers with objective and accurate measurements of the vascular structure of the CAM, such as the number of vessels and the vessel density of the membrane, which enables side-by-side comparison of different experiments.

WimCAM uses as input bright field microscopy images of the chorioallantoic membrane of chicken eggs. Both images taken from within the egg and images of a piece of the egg shell are suitable for the image analysis tool. Besides, our solution is engineered with the flexibility needed to fit other image kinds. So, if your CAM assay images do not fulfill the requirements above, don’t hesitate to contact us to get a trial for your images.