The use of the chorioallantoic membrane (CAM) of developing chicken eggs has become a key practice to study angiogenesis and vascular processes in many vascular and developmental biomedical investigations. The simplicity of the CAM assay and its similarity to in vivo models makes it an interesting alternative to study the growth and damage of vessels and capillaries in vascular structures, which constitutes a target step in the study of tumors, cancer, angiogenesis, vascular diseases, developmental biology and an extensive number of other investigations.

The concordance of the CAM assay results with the in vivo data turns it into an engaging alternative to observe the evolution of the vascular structures with the promise of representative results. The changes on the vessels of the CAM and modifications of its vascular structure can be easily observed by the human eye; however, the quantification of those changes in an objective and reproducible way is only possible using image processing techniques. WimCAM is conceived to quantify CAM assay images automatically with precision and reliability.

WimCAM provides biological and biomedical researchers with objective and accurate measurements of the vascular structure of the CAM, such as the number of vessels and the vessel density of the membrane, which enables side-by-side comparison of different experiments.

WimCAM uses as input bright field microscopy images of the chorioallantoic membrane of chicken eggs. Both images taken from within the egg and images of a piece of the egg shell are suitable for the image analysis tool. Besides, our solution is engineered with the flexibility needed to fit other image kinds. So, if your CAM assay images do not fulfill the requirements above, don’t hesitate to contact us to get a trial for your images.

There are many advantages of adding WimCAM to your workflow:

It is easy to use, fast and automated. Just upload your images and get your results in minutes.
Just pay for your number of images, not a cent more. WimCAM is a pay-per-use service.
It is able to work with many different types of microscopy techniques such as epifluorescence and confocal microscopy in adherent cell lines. If your image type is not covered, a custom solution can be developed upon request.
Takes objective measurements with precision and accuracy.
Suits for the reproducibility paradigm: same rules to measure the same kind of experiments.
Check your results from your Wimasis account anytime, anywhere. All you need is an Internet connection.

That is simple! Just go to myWim, Wimasis' Image Analysis Platform, and follow these steps:

1

Create an account

2

Upload your images

3

Enjoy the results

Would you like to know more about Wimasis Cloud? Please, click here for further information.

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Here you can find some extra resources that will help you to fully understand this solution: