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Monitoring autophagosome formation is essential for autophagy characterization.

Go automatic with WimAutophagy.
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Objective, reproducible, and precise analysis of autophagy assay images is ensured by our WimAutophagy online automated solution.

It is specifically designed to quantitatively monitor the autophagy process, by detecting the cell nuclei autophagosomes in the cytoplasm from cell or tissue cultures images.

You take the images, we do the rest.

Analyzing the autophagy assay

Autophagy represents the major process for degradation of aggregated cellular proteins and dysfunctional organelles, so alterations in autophagy are involved in the pathogenesis of many diseases, like nervous system disorders.

Thanks to our high-end image processing algorithms, WimAutophagy detects cell nuclei and allocates the autophagosomes to the corresponding nuclei, in order to obtain quantitative data per individual cell. It is also optimized to distinguish between isolated and clustered autophagosomes, in order to be able to estimate the number of autophagosomes and puncta per cell.

Discover the benefits of using WimAutophagy

There are many advantages of adding WimAutophagy to your workflow:

  • Easy to use, fast and automated. Just upload your images and get your results in minutes.
  • Just pay for your number of images, not a cent more. WimAutophagy is a pay-per-use service.
  • It is able to work with many different types of microscopy techniques such as epifluorescence and confocal microscopy in adherent cell lines. If your image type is not covered, a custom solution can be developed upon request.
  • Takes objective measurements with precision and accuracy.
  • Suits for the reproducibility paradigm: same rules to measure the same kind of experiments.
  • Check your results from your Wimasis account anytime, anywhere. All you need is an Internet connection.

How it works

That is simple! Just go to myWim, Wimasis' Image Analysis Platform, and follow these steps:

join us
Create an account
upload images
Upload your images
Enjoy the results

Would you like to know more about myWim? Please, click here for further information.

Results that make a difference

For every image that you upload, WimAutophagy will generate an output CSV sheet file that will give you insight into the outcome of your experiment. Some of the measurements included are:

  • Number of cells
  • Estimated autophagosome area and mean intensity
  • Mean number of puncta and autophagosomes per cell
  • Mean Nucleus area
  • Area of nucles, citoplasm and autophagosomes
  • Number of autophagosomes, by quantifying isolated and estimating clusters population

Besides, a detailed control image is generated in order to help you to visualize the parameters that WimAutophagy provides you with:

Negative cells
Positive Cells

Would you like to get any other parameter? Ask for it!

See more about WimAutophagy

Here you can find some extra resources that will help you to fully understand this solution:

Lucía García Ledo
Role of autophagy in neurodegeneration and new for its study
PhD Thesis - Complutense University of Madrid
September 2015
View all
case study
Centro de Investigaciones Biológicas (CIB-CSIC)
Centro de Investigaciones Biológicas (CIB-CSIC) ES
Wimasis is wondeful since we no longer have to spend our valuable time counting dots inside cells!
Dr. Patricia Boya
View case study
pie circulos


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