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Go automatic with WimAutophagy.
Objective, reproducible, and precise analysis of autophagy assay images is ensured by our WimAutophagy web-based automated solution.
It is specifically designed to quantitatively monitor the autophagy process, by detecting the cell nuclei autophagosomes in the cytoplasm from cell or tissue cultures images.
You take the images, we do the rest.
Autophagy represents the major process for degradation of aggregated cellular proteins and dysfunctional organelles, so alterations in autophagy are involved in the pathogenesis of many diseases, like nervous system disorders.
Thanks to our high-end image processing algorithms, WimAutophagy detects cell nuclei and allocates the autophagosomes to the corresponding nuclei, in order to obtain quantitative data per individual cell. It is also optimized to distinguish between isolated and clustered autophagosomes, in order to be able to estimate the number of autophagosomes and puncta per cell.
There are many advantages of adding WimAutophagy to your workflow:
- Easy to use, fast and automated. Just upload your images and get your results in minutes.
- Just pay for your number of images, not a cent more. WimAutophagy is a pay-per-use service.
- It is able to work with many different types of microscopy techniques such as epifluorescence and confocal microscopy in adherent cell lines. If your image type is not covered, a custom solution can be developed upon request.
- Takes objective measurements with precision and accuracy.
- Suits for the reproducibility paradigm: same rules to measure the same kind of experiments.
- Check your results from your Wimasis account anytime, anywhere. All you need is an Internet connection.
That is simple! Just go to myWim, Wimasis' Image Analysis Platform, and follow these steps:
Would you like to know more about myWim? Please, click here for further information.
For every image that you upload, WimAutophagy will generate an output CSV sheet file that will give you insight into the outcome of your experiment. Some of the measurements included are:
- Number of cells
- Estimated autophagosome area and mean intensity
- Mean number of puncta and autophagosomes per cell
- Mean Nucleus area
- Area of nucles, citoplasm and autophagosomes
- Number of autophagosomes, by quantifying isolated and estimating clusters population
Besides, a detailed control image is generated in order to help you to visualize the parameters that WimAutophagy provides you with:
Would you like to get any other parameter? Ask for it!
Here you can find some extra resources that will help you to fully understand this solution:
